Project:
* Name: Sciex_BVP_ProteoBench_05152025
* Description: 
* Project Version: 13 20250515
* Created at: 2025-05-16 15:39:16
+ Peptide FDR 1 max var mod
  Description: 
  PEAKS Version: 13 20250515
  Created at: 2025-05-16 17:28:34

  DIA Db Search Parameters:
    Precursor Mass Error Tolerance: Auto Detected
    Fragment Mass Error Tolerance: Auto Detected
    Match Between Run: Yes
    Enzyme: Specified by each sample
    Max Missed Cleavage: 1
    Digest Mode: Specific
    Max Variable PTM per Peptide: 1
    Fixed Modifications: 
    - Carbamidomethylation (+57.02)
    Variable Modifications: 
    - Acetylation (Protein N-term) (+42.01)
    - Oxidation (M) (+15.99)
    Database: 
    - ProteoBench_HYE
      - Taxonomy: all species
      - Searched Entries: 31889
     Peptide Length between: 6,30
     Precursor M/Z between: 380,980
     Fragment M/Z between: 150,2000
     Precursor Charge between: 1,5
    Find Sequence Variants: No
    Find Novel Peptides: No
    Report Filter: 
      Peptide FDR: 1.0%
      Proteins Unique Peptides >= 1

  LFQ Parameters:
    Quantification Type: Label Free Quantification
    LFQ Method: Identification Directed Quantification
    Retention Time Range: [0.0 - Max]
    Base Sample: LFQ_Astral_DIA_15min_50ng_Condition_A_REP1_raw
    Peptide Feature Filter:
      Avg Area >= 0.0
      Quality >= 0.0
      Charge between: [1 - 5]
      Detected in at least 0 samples per group
      Use in Group Coefficient of Variation Filter: No
    Protein:
      Significance Method: ANOVA
      Modified Form Exclusion: Yes
      Remove Outlier: Yes
      Use Top 3 Peptide
      Significance >= 0.0
      Fold Change between: [1.0 - 64.0]
      Has at least 1 Used Peptide
    Samples: 6 Samples in 2 Groups
      Group 1: 
        LFQ_Astral_DIA_15min_50ng_Condition_A_REP1_raw;LFQ_Astral_DIA_15min_50ng_Condition_A_REP2_raw;LFQ_Astral_DIA_15min_50ng_Condition_A_REP3_raw;
      Group 2: 
        LFQ_Astral_DIA_15min_50ng_Condition_B_REP1_raw;LFQ_Astral_DIA_15min_50ng_Condition_B_REP2_raw;LFQ_Astral_DIA_15min_50ng_Condition_B_REP3_raw;
    Normalization Method: No Normalization

