Project:
* Name: 250522_PEAKS13_Astral_DIA_50ng_15min
* Description: 
* Project Version: 13 20250520
* Created at: 2025-05-22 17:18:52
+ Proteobench-Trypsin/P
  Description: 
  PEAKS Version: 13 20250520
  Created at: 2025-07-07 16:59:56

  DIA Db Search Parameters:
    Precursor Mass Error Tolerance: Auto Detected
    Fragment Mass Error Tolerance: Auto Detected
    Match Between Run: Yes
    Enzyme: Trypsin/P
    Max Missed Cleavage: 1
    Digest Mode: Specific
    Max Variable PTM per Peptide: 1
    Fixed Modifications: 
    - Carbamidomethylation (+57.02)
    Variable Modifications: 
    - Oxidation (M) (+15.99)
    - Acetylation (Protein N-term) (+42.01)
    Database: 
    - Proteobench
      - Taxonomy: all species
      - Searched Entries: 31889
     Peptide Length between: 6,30
     Precursor M/Z between: 400,1000
     Fragment M/Z between: 50,2000
     Precursor Charge between: 1,5
    Find Sequence Variants: No
    Find Novel Peptides: No
    Report Filter: 
      Peptide FDR: 1.0%
      Precursor FDR: 1.0%
      Proteins Unique Peptides >= 1

  LFQ Parameters:
    Quantification Type: Label Free Quantification
    LFQ Method: Identification Directed Quantification
    Retention Time Range: [0.0 - Max]
    Base Sample: Average
    Peptide Feature Filter:
      Avg Area >= 0.0
      Quality >= 0.0
      Charge between: [1 - 5]
      Detected in at least 0 samples per group
      Use in Group Coefficient of Variation Filter: No
    Protein:
      Significance Method: ANOVA
      Modified Form Exclusion: Yes
      Remove Outlier: Yes
      Use Top 3 Peptide
      Significance >= 0.0
      Fold Change between: [1.0 - 64.0]
      Has at least 1 Used Peptide
    Samples: 6 Samples in 2 Groups
      Condition A: 
        LFQ_Astral_DIA_15min_50ng_Condition_A_REP1;LFQ_Astral_DIA_15min_50ng_Condition_A_REP2;LFQ_Astral_DIA_15min_50ng_Condition_A_REP3;
      Condition B: 
        LFQ_Astral_DIA_15min_50ng_Condition_B_REP1;LFQ_Astral_DIA_15min_50ng_Condition_B_REP2;LFQ_Astral_DIA_15min_50ng_Condition_B_REP3;
    Normalization Method: No Normalization

